CRISPR editing in organoids
Accepted for publication in Cell Reports Medicine, the article "Prime editing functionally corrects cystic fibrosis-causing CFTR mutations in human organoids and airway epithelial cells" from the research group of professor Marianne Carlon, demonstrates how organoids can elegantly be used to evaluate efficacy of gene therapies in human tissue derived in vitro models.
https://lnkd.in/dN6UdA-N
Some Highlights:
*Prime editing can be used to precisely correct cystic fibrosis-causing mutations
*Efficient functional correction in patient-derived organoids and airway epithelium
*Machine learning tool DETECTOR functionally screens genetic strategies in primary organoids
*Genome-wide evaluation of prime editing for L227R and N1303K confirms high fidelity
The paper received considerable interest in lay media:
